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KMID : 0616120200510020139
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Korean Journal of Pharmacognosy
2020 Volume.51 No. 2 p.139 ~ p.145
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Simultaneous Quantitative Analysis of Flavonoids Isolated from the Leaves of Diospyros kaki
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Kim Ga-Ram
Kim Eun-Nam
Jeong Gil-Saeng
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Abstract
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The leaves of Diospyros kaki Thunb. were used to treat heart disease and hypotension in traditionally East Asia. The purpose of this study is to simultaneously quantitiative analyze the content of flavonoids in leaves of D. kaki. The isolated flavonoids from the leaves of D. kaki, and the structure of the isolated flavonoids were analyzed based on 1H and 13C NMR spectrum compared to literature data. Simultaneous quantitative analysis of the isolated flavonoids was validated using high performance liquid chromatography (HPLC). Results showed that the calibration curves of the flavonoid compounds were confirmed that they have a large linearity with a correlation coefficient (R2) of 0.99. In the intra-day and inter-day analysis, accuracy and precision of five compounds were measured that accuracy was 94.39 to 114.47% and precision was less than 3.00%. Content analysis showed hyperoside (1.30 ± 0.09%), astragalin (0.81 ± 0.06%), trifoline (1.58 ± 0.07%), quercetin (0.13 ± 0.02%) and kaempferol (1.33 ± 0.25%).
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KEYWORD
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HPLC, Flavonoid, Quantitative analysis, Diospyros kaki Thunb
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